Anticorps Monoclonal anti-Calbindin-D28k

Calbindin-D28k Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1F8B9

N° de cat : 66394-1-Ig

Synonymes

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

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Galerie de données de validation

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  WB analysis using 66394-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66394-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66394-1-Ig

  Human brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat cerebellum using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green).

• 

  IF Staining of PC-12 using 66394-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de lapin, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal, tissu de cervelet de lapin, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cervelet de rat, tissu cérébral humain, tissu d'intestin grêle humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cervelet de souris, cellules PC-12, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:8000-1:32000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 7 publications below

Informations sur le produit

66394-1-Ig cible Calbindin-D28k dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, porc, rat, souris

• Réactivité: Humain, Lapin, porc, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calbindin-D28k Protéine recombinante Ag5861
• Nom complet: calbindin 1, 28kDa
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC006478
• Symbole du gène: CALB1
• Identification du gène (NCBI): 793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); Integrative Single-Cell Transcriptomics and Epigenomics Mapping of the Fetal Retina Developmental Dynamics; Authors - Ruonan Li
mouse	IF	Cell Death Differ; The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.; Authors - Fei Wang
mouse	IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	IF	Int Immunopharmacol; Clinical significance of Interleukin 17 receptor E in diabetic nephropathy; Authors - Jingjing Wang
rat	IF	Front Neurosci; Rodent Area Prostriata Converges Multimodal Hierarchical Inputs and Projects to the Structures Important for Visuomotor Behaviors.; Authors - Chang-Hui Chen
rat	IF	Neurosci Lett; Retinoic acid supplementation ameliorates motor incoordination via RARα-CBLN2 in the cerebellum of a prenatal valproic acid-exposed rat autism model; Authors - Binlin Yuan