Anticorps Polyclonal de lapin anti-Calmegin

Calmegin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12629-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12629-1-AP (same clone as 12629-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12629-1-AP (same clone as 12629-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12629-1-AP (same clone as 12629-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12629-1-AP (same clone as 12629-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

  IP result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12629-1-PBS cible Calmegin dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calmegin Protéine recombinante Ag3322
• Nom complet: calmegin
• Masse moléculaire calculée: 610 aa, 70 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC028357
• Symbole du gène: Calmegin
• Identification du gène (NCBI): 1047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

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