Anticorps Monoclonal anti-Calnexin

• Phare
• Validé par KD/KO

Calnexin Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(82)

CloneNo.

2A2C6

Avis (2)

N° de cat : 66903-1-Ig

Synonymes

CANX, 2A2C6, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88

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Galerie de données de validation

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  WB analysis of HEK-293 using 66903-1-Ig

  WB result of Calnexin antibody (66903-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells.

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  WB analysis using 66903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66903-1-Ig

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 66903-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 66903-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 59 publications below
• IHC: See 1 publications below
• IF: See 27 publications below

Informations sur le produit

66903-1-Ig cible Calnexin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calnexin Protéine recombinante Ag25535
• Nom complet: calnexin
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC003552
• Symbole du gène: Calnexin
• Identification du gène (NCBI): 821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Publications

Species	Application	Title
human	IF	Cell Res; Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria; Authors - Xiao-Qing Xie
mouse,human	WB,IF	Nat Commun; ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy; Authors - Xiangling Xiao
human	WB	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty
mouse	IF	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
rat	WB	J Control Release; Extracellular vesicles encapsulated with caspase-1 inhibitor ameliorate experimental autoimmune myasthenia gravis through targeting macrophages; Authors - Yang Zhou
mouse	IF	Clin Transl Med; Targeting KDM4 family epigenetically triggers antitumour immunity via enhancing tumour-intrinsic innate sensing and immunogenicity; Authors - Mayu Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Samantha (Verified Customer) (03-18-2024)

Tested on HeLA cells and produced clean bands that were cut out and analysed with Mass Spectrometry

• Applications: Western Blot
• Primary Antibody Dilution: 1/10,000
• Cell Tissue Type: HeLa Cells

FH

Amy (Verified Customer) (11-08-2023)

Clean antibody with strong band for WB in HEK293T lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: HEK293T

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