Anticorps Recombinant de lapin anti-Calnexin

Calnexin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3K18

N° de cat : 81938-1-PBS

Synonymes

CANX, 3K18, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88

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Galerie de données de validation

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  WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 81938-1-PBS

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81938-1-PBS (Calnexin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81938-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81938-1-PBS cible Calnexin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Calnexin Protéine recombinante Ag0697
• Nom complet: calnexin
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC003552
• Symbole du gène: Calnexin
• Identification du gène (NCBI): 821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

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