Anticorps Polyclonal de lapin anti-Calpain 11

Calpain 11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13675-1-PBS

Synonymes

EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11

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Galerie de données de validation

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  WB analysis of PC-3 using 13675-1-AP (same clone as 13675-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13675-1-AP (same clone as 13675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13675-1-AP (same clone as 13675-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpain 11 antibody (13675-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13675-1-PBS cible Calpain 11 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 11 Protéine recombinante Ag4668
• Nom complet: calpain 11
• Masse moléculaire calculée: 702 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC033733
• Symbole du gène: Calpain 11
• Identification du gène (NCBI): 11131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.

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