Anticorps Monoclonal anti-Calponin

Calponin Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11E5

N° de cat : 66540-1-PBS

Synonymes

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

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Galerie de données de validation

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  WB analysis using 66540-1-Ig (same clone as 66540-1-PBS)

  Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50017-1

  Sandwich ELISA standard curve ofMP50017-1, Calponin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66540-2-PBS. Detection antibody: HRP-conjugated 66540-1-PBS. Standard: Ag21384. Range: 0.313-20 ng/mL.

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  IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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  IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66540-1-PBS cible Calponin dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calponin Protéine recombinante Ag21384
• Nom complet: calponin 1, basic, smooth muscle
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC022015
• Symbole du gène: Calponin
• Identification du gène (NCBI): 1264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

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