Anticorps Monoclonal anti-Calretinin

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D7A9

N° de cat : 66496-1-PBS

Synonymes

CALB2, 29 kDa calbindin, 2D7A9, CAB29, CAL2

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Galerie de données de validation

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  WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66496-1-Ig (same clone as 66496-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66496-1-Ig (same clone as 66496-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66496-1-Ig (same clone as 66496-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 66496-1-Ig (same clone as 66496-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50295-3

  Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.

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Informations sur le produit

66496-1-PBS cible Calretinin dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calretinin Protéine recombinante Ag2924
• Nom complet: calbindin 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC015484
• Symbole du gène: Calretinin
• Identification du gène (NCBI): 794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

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