Anticorps Monoclonal anti-Calsequestrin 2

Calsequestrin 2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C10A1

N° de cat : 66419-1-PBS

Synonymes

Calsequestrin 2, CASQ2, PDIB2

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Galerie de données de validation

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  WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 66419-1-Ig (same clone as 66419-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 66419-1-Ig (same clone as 66419-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66419-1-PBS cible Calsequestrin 2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Calsequestrin 2 Protéine recombinante Ag13246
• Nom complet: calsequestrin 2 (cardiac muscle)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC022288
• Symbole du gène: Calsequestrin 2
• Identification du gène (NCBI): 845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

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