Anticorps Monoclonal anti-Cardiac Troponin T

Cardiac Troponin T Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E2D8

N° de cat : 68300-1-PBS

Synonymes

TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8

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Galerie de données de validation

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  WB analysis of pig heart using 68300-1-Ig (same clone as 68300-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  WB analysis of chicken heart using 68300-1-Ig (same clone as 68300-1-PBS)

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50338-1

  Cytometric bead array standard curve of MP50338-1, cTnT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68300-1-PBS. Detection antibody: 68300-2-PBS. Standard:Ag7716. Range: 0.391-50 ng/mL.

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Informations sur le produit

68300-1-PBS cible Cardiac Troponin T dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cardiac Troponin T Protéine recombinante Ag7716
• Nom complet: troponin T type 2 (cardiac)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC002653
• Symbole du gène: Cardiac Troponin T
• Identification du gène (NCBI): 7139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

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