Anticorps Polyclonal de lapin anti-Cas9

Cas9 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Bactérie et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 26758-1-AP

Synonymes

dCas9, nCas9, Cas9 7A9, CRISPR, CRISPR/Cas9

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Galerie de données de validation

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  WB analysis of HeLa using 26758-1-AP

  HeLa cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 26758-1-AP

  A431 cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26758-1-AP

  IP result of anti-Cas9 (IP:26758-1-AP, 4ug; Detection:26758-1-AP 1:1000) with HeLa cells transfected with CRISPR-Cas9 600 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

26758-1-AP cible Cas9 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Bactérie

• Réactivité: Bactérie
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cas9 Protéine recombinante Ag25281
• Nom complet: Cas9
• Masse moléculaire calculée: 160 kDa
• Numéro d’acquisition GenBank: NP_269215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.
This antibody can recognize Cas9 protein with point mutations, such as dCas9 and nCas9.

Publications

Species	Application	Title
	WB	Nature; Enhancer release and retargeting activates disease-susceptibility genes.; Authors - Soohwan Oh
human	WB	Adv Sci (Weinh); Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression; Authors - Xiaosong Han
human	WB	Clin Epigenetics; E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis; Authors - Jingxuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kiara (Verified Customer) (07-28-2025)

This primary antibody is not validated for IF, but I gave it a shot anyways. As expected, it did not give a reliable signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: iPSC-derived cardiomyocytes

Cas9 Antibody Immunofluorescence validation (1:400 dilution) in iPSC-derived cardiomyocytes (Cat no:26758-1-AP)

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