Anticorps Polyclonal de lapin anti-Casein Kinase 1 Delta

• Phare
• Validé par KD/KO

Casein Kinase 1 Delta Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14388-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 14388-1-AP (same clone as 14388-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14388-1-AP (same clone as 14388-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14388-1-AP (Casein Kinase 1 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 14388-1-AP (same clone as 14388-1-PBS)

  IP result of anti-Casein Kinase 1 Delta (IP:14388-1-AP, 3ug; Detection:14388-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14388-1-AP (same clone as 14388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14388-1-AP (same clone as 14388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14388-1-AP (Casein Kinase 1 Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14388-1-AP (same clone as 14388-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 14388-1-AP (same clone as 14388-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using Casein Kinase 1 Delta antibody (14388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14388-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14388-1-PBS cible Casein Kinase 1 Delta dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Casein Kinase 1 Delta Protéine recombinante Ag5856
• Nom complet: casein kinase 1, delta
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC003558
• Symbole du gène: CK1 Delta
• Identification du gène (NCBI): 1453
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

{{ptg:RelatedPrimaryAntibodies}}