Anticorps Polyclonal de lapin anti-Caspase 1/P20

• Phare
• Validé par KD/KO

Caspase 1/P20 Polyclonal Antibody for WB, IHC, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 22915-1-PBS

Synonymes

CASP1, Caspase1, CASP 1, CASP-1, Caspase 1

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Galerie de données de validation

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  WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 22915-1-AP (same clone as 22915-1-PBS)

  WB result of Caspase 1/P20 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

• 

  IF Staining using 22915-1-AP (same clone as 22915-1-PBS)

  IF result of 22915-1-AP from publication with PMID: 31611559. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22915-1-PBS cible Caspase 1/P20 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 1/P20 Protéine recombinante Ag11143
• Nom complet: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Masse moléculaire calculée: 404 aa, 45 kDa
• Poids moléculaire observé: 45-47 kDa, 30 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC062327
• Symbole du gène: Caspase 1
• Identification du gène (NCBI): 834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.

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