Anticorps Recombinant de lapin anti-Caspase 10

Caspase 10 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242353H6

N° de cat : 85196-3-PBS

Synonymes

CASP10, Caspase10, 242353H6, Apoptotic protease Mch 4, Apoptotic protease Mch-4

---

Galerie de données de validation

• 

  WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

• 

  IHC staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85196-3-RR (Caspase 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 85196-3-RR (same clone as 85196-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 10 antibody (85196-3-RR, Clone: 242353H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 85196-3-RR (same clone as 85196-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-3-RR, Clone:242353H6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

---

Informations sur le produit

85196-3-PBS cible Caspase 10 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 10, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC042844
• Symbole du gène: Caspase 10
• Identification du gène (NCBI): 843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.

{{ptg:RelatedPrimaryAntibodies}}