Anticorps Recombinant de lapin anti-Caspase 3/P17/P19

• Phare
• Validé par KD/KO

Caspase 3/P17/P19 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

5G20

N° de cat : 82202-1-PBS

Synonymes

CASP3, Caspase3, 5G20, CASP 3, CASP-3

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Galerie de données de validation

• 

  WB analysis of Jurkat using 82202-1-RR (same clone as 82202-1-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82202-1-RR (same clone as 82202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

  WB result of Caspase 3/P17/P19 antibody (82202-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3/P17/P19 transfected HeLa cells. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82202-1-RR (same clone as 82202-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3/P17/19 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

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Applications publiées

• IF: See 1 publications below

Informations sur le produit

82202-1-PBS cible Caspase 3/P17/P19 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-35 kDa, 17 kDa, 19 kDa
• Numéro d’acquisition GenBank: NM_004346
• Symbole du gène: Caspase 3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
mouse	IF	mSystems; Multi-omics investigation of Porphyromonas gingivalis exacerbating acute kidney injury through the gut-kidney axis; Authors - Ling Dong

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