Anticorps Monoclonal anti-Caspase 3/P17/P19

• Phare
• Validé par KD/KO

Caspase 3/P17/P19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2G4B2

N° de cat : 66470-2-PBS

Synonymes

CASP3, Caspase3, 2G4B2, CASP 3, CASP-3

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Galerie de données de validation

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  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3/P17/P19 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3/P17/P19 transfected HeLa cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66470-2-Ig (same clone as 66470-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig (same clone as 66470-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 3/p17/p19 antibody (66470-2-Ig, Clone: 2G4B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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Informations sur le produit

66470-2-PBS cible Caspase 3/P17/P19 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 3/P17/P19 Protéine recombinante Ag25029
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 277 aa, 32 kDa
• Poids moléculaire observé: 32-35 kDa, 19 kDa, 17 kDa
• Numéro d’acquisition GenBank: BC016926
• Symbole du gène: Caspase 3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

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