Anticorps Monoclonal anti-Caspase 6

• Phare
• Validé par KD/KO

Caspase 6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H7F9

N° de cat : 67825-1-PBS

Synonymes

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

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Galerie de données de validation

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  WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

  WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67825-1-PBS cible Caspase 6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 6 Protéine recombinante Ag20534
• Nom complet: caspase 6, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 33 kDa, 22 kDa
• Poids moléculaire observé: 33-35 kDa，22kDa, 18kDa
• Numéro d’acquisition GenBank: BC000305
• Symbole du gène: Caspase 6
• Identification du gène (NCBI): 839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

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