Anticorps Recombinant de lapin anti-Caspase 6

Caspase 6 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242457A8

N° de cat : 85201-2-PBS

Synonymes

CASP6, Caspase6, 242457A8, Apoptotic protease Mch 2, Apoptotic protease Mch-2

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Galerie de données de validation

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  WB analysis of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85201-2-RR (Caspase 6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85201-2-RR (Caspase 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using Caspase 6 antibody (85201-2-RR, Clone: 242457A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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Informations sur le produit

85201-2-PBS cible Caspase 6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 6, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 33 kDa, 22 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000305
• Symbole du gène: Caspase 6
• Identification du gène (NCBI): 839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons.

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