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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Caspase 7

Caspase 7 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(71)

N° de cat : 27155-1-AP

Synonymes

CASP7, Apoptotic protease Mch 3, Apoptotic protease Mch-3, CASP 7, CASP-7



Applications testées

Résultats positifs en WBcellules Jurkat, cellules HEK-293, cellules LNCaP, cellules MCF-7, cellules PC-3, tissu hépatique de souris
Résultats positifs en IPcellules HEK-293,
Résultats positifs en IF-Ptissu cérébral de souris,
Résultats positifs en IF/ICCcellules MCF-7

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

27155-1-AP cible Caspase 7 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, bovin, Humain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Caspase 7 Protéine recombinante Ag25904
Nom complet caspase 7, apoptosis-related cysteine peptidase
Masse moléculaire calculée 303 aa, 34 kDa
Poids moléculaire observé 35 kDa
Numéro d’acquisition GenBankBC015799
Symbole du gène Caspase 7
Identification du gène (NCBI) 840
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Protocole

Product Specific Protocols
WB protocol for Caspase 7 antibody 27155-1-APDownload protocol
IF protocol for Caspase 7 antibody 27155-1-APDownload protocol
IP protocol for Caspase 7 antibody 27155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanWB

J Pineal Res

Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.

Authors - Yue Gao
humanWB

Cell Rep

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation

Authors - Zehe Ge
humanWB

Front Immunol

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma

Authors - Jinfeng Zhu
humanWB

Phytother Res

Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT

Authors - Xiaoli Li
humanWB

Cell Death Discov

IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progression

Authors - Qianting Tian
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