Anticorps Monoclonal anti-Caspase 7

Caspase 7 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C9H4

N° de cat : 67956-1-Ig

Synonymes

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

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Galerie de données de validation

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  WB analysis using 67956-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 67956-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 67956-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'estomac de rat, tissu de cancer du foie humain, tissu d'estomac de souris, tissu hépatique de rat, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67956-1-Ig cible Caspase 7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 7 Protéine recombinante Ag27601
• Nom complet: caspase 7, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 303 aa, 34 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC015799
• Symbole du gène: CASP7
• Identification du gène (NCBI): 840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.