Anticorps Recombinant de lapin anti-Cleaved Caspase 8/P18

Cleaved Caspase 8/P18 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242445E5

N° de cat : 85171-3-PBS

Synonymes

CASP8, Caspase 8, 242445E5, Apoptotic cysteine protease, Apoptotic protease Mch-5

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Galerie de données de validation

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  WB analysis using 85171-3-RR (same clone as 85171-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85171-3-RR (Cleaved Caspase 8/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85171-3-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 85171-3-RR (same clone as 85171-3-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85171-3-RR (Cleaved Caspase 8/P18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85171-3-PBS in a different storage buffer formulation.

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Informations sur le produit

85171-3-PBS cible Cleaved Caspase 8/P18 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 8, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 538 aa, 62 kDa
• Poids moléculaire observé: 55 kDa, 18 kDa
• Numéro d’acquisition GenBank: BC028223
• Symbole du gène: Caspase 8
• Identification du gène (NCBI): 841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). This antibody recognize the cleaved caspase 8.

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