Anticorps Monoclonal anti-Caspase 8/P43/P18

• Phare
• Validé par KD/KO

Caspase 8/P43/P18 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B9H8

N° de cat : 66093-1-PBS

Synonymes

CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5

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Galerie de données de validation

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  WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)

  WB result of Caspase 8/P43/P18 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

  IP result of anti-Caspase 8/P43/P18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 8/P43/P18 antibody (66093-1-Ig, Clone: 2B9H8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66093-1-PBS cible Caspase 8/P43/P18 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 8/P43/P18 Protéine recombinante Ag20524
• Nom complet: caspase 8, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 538 aa, 62 kDa
• Poids moléculaire observé: 53-57 kDa, 32-45 kDa, 18 kDa
• Numéro d’acquisition GenBank: BC028223
• Symbole du gène: Caspase 8
• Identification du gène (NCBI): 841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

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