Anticorps Recombinant de lapin anti-Caspr2/CNTNAP2

Caspr2/CNTNAP2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242391A9

N° de cat : 85116-4-RR

Synonymes

CNTNAP2, 242391A9, CASPR2, Contactin-associated protein-like 2

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Galerie de données de validation

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  WB analysis using 85116-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85116-4-RR (CNTNAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85116-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85116-4-RR against Human Caspr2/CNTNAP2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85116-4-RR cible Caspr2/CNTNAP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Caspr2/CNTNAP2 Protéine recombinante Ag22844
• Nom complet: contactin associated protein-like 2
• Masse moléculaire calculée: 1331 aa, 148 kDa
• Poids moléculaire observé: 148 kDa
• Numéro d’acquisition GenBank: BC113373
• Symbole du gène: Grancalcin
• Identification du gène (NCBI): 26047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspr2 is a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons, and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2, a transcription factor related to speech and language development.

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