Anticorps Monoclonal anti-Catalase

• Phare
• Validé par KD/KO

Catalase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F8D7

N° de cat : 66765-1-PBS

Synonymes

CAT, 1F8D7, EC:1.11.1.6

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Galerie de données de validation

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  WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66765-1-Ig (same clone as 66765-1-PBS)

  WB result of Catalase antibody (66765-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66765-1-Ig (same clone as 66765-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66765-1-Ig (same clone as 66765-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Catalase antibody (66765-1-Ig, Clone: 1F8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66765-1-PBS cible Catalase dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Catalase Protéine recombinante Ag16916
• Nom complet: catalase
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC112219
• Symbole du gène: Catalase
• Identification du gène (NCBI): 847
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

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