Anticorps Polyclonal de lapin anti-Cathepsin A

Cathepsin A Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15020-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  WB analysis of Sp2/0 using 15020-1-AP (same clone as 15020-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15020-1-PBS cible Cathepsin A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin A Protéine recombinante Ag7073
• Nom complet: cathepsin A
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60, 32, 20 kDa
• Numéro d’acquisition GenBank: BC000597
• Symbole du gène: Cathepsin A
• Identification du gène (NCBI): 5476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

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