Anticorps Recombinant de lapin anti-Cathepsin B

Cathepsin B Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

243020A5

N° de cat : 85841-5-RR

Synonymes

Cathepsin B heavy chain, Cathepsin B light chain, Cathepsin B1, Ctsb, EC:3.4.22.1

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Galerie de données de validation

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  WB analysis using 85841-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85841-5-RR (Cathepsin B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85841-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85841-5-RR (Cathepsin B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 85841-5-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cathepsin B antibody (85841-5-RR, Clone: 243020A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of RAW 264.7 using 85841-5-RR

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Cathepsin B Recombinant antibody (85841-5-RR, Clone:243020A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,
• Résultats positifs en FC (Intra): cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85841-5-RR cible Cathepsin B dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cathepsin B Protéine recombinante Eg3028
• Nom complet: cathepsin B
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 31 kDa, 39 kDa
• Numéro d’acquisition GenBank: NM_007798.3
• Symbole du gène: Ctsb
• Identification du gène (NCBI): 13030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 39 kDa form of pro-CTSB, 31 kDa mature forms in mouse brain by western blot.

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