Anticorps Polyclonal de lapin anti-Cathepsin C

Cathepsin C Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30790-1-PBS

Synonymes

CTSC, CPPI

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Galerie de données de validation

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  WB analysis using 30790-1-AP (same clone as 30790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30790-1-AP (Cathepsin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30790-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 30790-1-AP (same clone as 30790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30790-1-AP (Cathepsin C antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30790-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30790-1-AP (same clone as 30790-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Cathepsin C antibody (30790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30790-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30790-1-PBS cible Cathepsin C dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin C Protéine recombinante Ag32420
• Nom complet: cathepsin C
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_001814
• Symbole du gène: Cathepsin C
• Identification du gène (NCBI): 1075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cathepsin C, also known as DPP-I. It is expected to be located in lysosome and highly expressed in lung, kidney and placenta (PMID: 9092576). This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. The calculated molecular weight of Cathepsin C is 51 kDa. The 23-kDa band may be a subunit of the protein monomer, while a 100-kda band is a dimer form (PMID: 26884336, 31557781).

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