Anticorps Polyclonal de lapin anti-Cathepsin D

• Phare
• Validé par KD/KO

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21327-1-PBS

Synonymes

CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD

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Galerie de données de validation

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  WB analysis of HCT 116 using 21327-1-AP (same clone as 21327-1-PBS)

  WB result of Cathepsin D antibody (21327-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and sg-Cathepsin D transfected HCT-116 cells. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 21327-1-AP (same clone as 21327-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 21327-1-AP (same clone as 21327-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21327-1-PBS cible Cathepsin D dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin D Protéine recombinante Ag15254
• Nom complet: cathepsin D
• Masse moléculaire calculée: 412 aa, 45 kDa
• Poids moléculaire observé: 32 kDa, 48 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC016320
• Symbole du gène: Cathepsin D
• Identification du gène (NCBI): 1509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

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