Anticorps Recombinant de lapin anti-Cathepsin D

Cathepsin D Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240775E7

N° de cat : 83743-2-PBS

Synonymes

CTSD, 240775E7, Cathepsin D heavy chain, Cathepsin D light chain, CLN10

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Galerie de données de validation

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  WB analysis using 83743-2-RR (same clone as 83743-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00695-4

  Sandwich ELISA standard curve of MP00695-4, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 83743-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1053. 83743-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00695-4

  Saliva of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 139.6 ng/mL with a range of 102.4 - 182.3 ng/mL.

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  Sample test of MP00695-4

  Human milk of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 580.4 ng/mL with a range of 270 - 1,071.8 ng/mL.

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  Sample test of MP00695-4

  For the A431 supernatant, the mean human Cathepsin D concentration was determined to be 301.05 ng/mL.

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  Sample test of MP00695-4

  Serum of sixteen individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 147.3 ng/mL with a range of 43.2 - 224.2 ng/mL.

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  IF Staining of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (83743-2-RR, Clone: 240775E7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FLNA antibody (67133-1-Ig, Clone: 1G4H3, red). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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Informations sur le produit

83743-2-PBS cible Cathepsin D dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cathepsin D Protéine recombinante Eg1053
• Nom complet: cathepsin D
• Masse moléculaire calculée: 45kDa
• Poids moléculaire observé: 32 kDa, 48 kDa, 52 kDa
• Numéro d’acquisition GenBank: NM_001909.5
• Symbole du gène: Cathepsin D
• Identification du gène (NCBI): 1509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

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