Anticorps Recombinant de lapin anti-Cathepsin D

Cathepsin D Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240775G2

N° de cat : 83743-5-RR

Synonymes

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

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Galerie de données de validation

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  WB analysis using 83743-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83743-5-RR (Cathepsin D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83743-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cathepsin D antibody (83743-5-RR, Clone: 240775G2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83743-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83743-5-RR against Human Cathepsin D were performed. The affinity constant is 0.821 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83743-5-RR cible Cathepsin D dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cathepsin D
• Masse moléculaire calculée: 45kDa
• Poids moléculaire observé: 32 kDa, 48 kDa, 52 kDa
• Numéro d’acquisition GenBank: NM_001909.5
• Symbole du gène: Cathepsin D
• Identification du gène (NCBI): 1509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

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