Anticorps Polyclonal de lapin anti-Caveolin-3

Caveolin-3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 28358-1-AP

Synonymes

CAV3, Caveolin3, M-caveolin, LQT9, LGMD1C

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Galerie de données de validation

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  WB analysis of mouse colon using 28358-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 28358-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 28358-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Caveolin-3 antibody (28358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de muscle squelettique de souris,
• Résultats positifs en IF/ICC: cellules H9C2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

28358-1-AP cible Caveolin-3 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caveolin-3 Protéine recombinante Ag28009
• Nom complet: caveolin 3
• Masse moléculaire calculée: 151 aa, 17 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC069368
• Symbole du gène: Caveolin-3
• Identification du gène (NCBI): 859
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	Oxid Med Cell Longev; Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor.; Authors - Lin Wang
mouse	WB	Cell Metab; Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining; Authors - Ya Liu
rat	WB,IF	Int Heart J; Decrease in Caveolae-Gαq Interaction Mediates Pressure Overload-Induced Cardiac Remodeling in Rats; Authors - Lijin Wang

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