Anticorps Recombinant de lapin anti-MCP-1/CCL2

MCP-1/CCL2 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240341G3

N° de cat : 83370-2-PBS

Synonymes

Ccl2, 240341G3, C-C motif chemokine 2, CCL 2, CCL-2

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Galerie de données de validation

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  WB analysis of NR8383 using 83370-2-RR (same clone as 83370-2-PBS)

  Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83370-2-RR (Ccl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83370-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00390-3

  Sandwich ELISA standard curve of MP00390-3, rat MCP-1/CCL-2 Recombinant Matched Antibody Pair - PBS only. 83370-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0397. 83370-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL..

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  Sample test of MP00390-3

  Serum of sixteen rat donors was measured. The MCP-1/CCL-2 concentration of detected samples was determined to be 8,981.3 pg/mL with a range of 5,770.2 - 16,068.9 pg/mL.

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  Sample test of MP00390-3

  Rat spleen cell cultures (1/2 spleen; 1-2 mm pieces in 50 mL DMEM supplemented with 10% fetal bovine serum, containing 100 ng/mL LPS) were collected after culturing for 3 days in a CO2-enriched (9.5%) incubator. The cell culture supernatant was assayed for rat MCP-1/CCL2 and measured 5,804.62 pg/mL.

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  Affinity and Kinetic Characterization of 83370-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83370-2-RR against Rat MCP-1 were performed. The affinity constant is 0.836 nM.

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Informations sur le produit

83370-2-PBS cible MCP-1/CCL2 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCP-1/CCL2 Protéine recombinante Eg0397
• Nom complet: chemokine (C-C motif) ligand 2
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: NM_031530
• Symbole du gène: MCP-1/CCL2
• Identification du gène (NCBI): 24770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

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