Anticorps Recombinant de lapin anti-MCP-1/CCL2

MCP-1/CCL2 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240341C1

N° de cat : 83370-4-PBS

Synonymes

Ccl2, C-C motif chemokine 2, CCL 2, CCL-2, chemokine (C C motif) ligand 2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00390-3

  Sandwich ELISA standard curve of MP00390-3, rat MCP-1/CCL-2 Recombinant Matched Antibody Pair - PBS only. 83370-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0397. 83370-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL..

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  Sample test of MP00390-3

  Rat spleen cell cultures (1/2 spleen; 1-2 mm pieces in 50 mL DMEM supplemented with 10% fetal bovine serum, containing 100 ng/mL LPS) were collected after culturing for 3 days in a CO2-enriched (9.5%) incubator. The cell culture supernatant was assayed for rat MCP-1/CCL2 and measured 5,804.62 pg/mL.

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  Sample test of MP00390-3

  Serum of sixteen rat donors was measured. The MCP-1/CCL-2 concentration of detected samples was determined to be 8,981.3 pg/mL with a range of 5,770.2 - 16,068.9 pg/mL.

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  Affinity and Kinetic Characterization of 83370-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83370-4-PBS against Rat MCP-1/CCL2 was performed. The affinity constant is 2.79 nM.

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Informations sur le produit

83370-4-PBS cible MCP-1/CCL2 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCP-1/CCL2 Protéine recombinante Eg0397
• Nom complet: chemokine (C-C motif) ligand 2
• Masse moléculaire calculée: 16 kDa
• Numéro d’acquisition GenBank: NM_031530
• Symbole du gène: MCP-1/CCL2
• Identification du gène (NCBI): 24770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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