Anticorps Recombinant de lapin anti-CD74

CD74 Recombinant Antibody for IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250109B11

N° de cat : 85802-2-PBS

Synonymes

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

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Galerie de données de validation

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  Sandwich ELISA sample test of MP02137-1

  RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD74, and measured 14.17 ng/mL.

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  Sandwich ELISA sample test of MP02137-1

  The mean CD74 concentration was determined to be 856.01 ng/mL in mouse thymus tissue extract based on a 5.80 mg/mL extract load and 789.39 ng/mL in mouse spleen tissue extract based on a 8.50 mg/mL extract load.

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  Sandwich ELISA sample test of MP02137-1

  Serum of sixteen mice was measured. The CD74 concentration of detected samples was determined to be 21.51 pg/mL with a range of 12.61-42.94 pg/mL.

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  Sandwich ELISA standard curve of MP02137-1

  Sandwich ELISA standard curve of MP02137-1, Mouse CD74 Recombinant Matched Antibody Pair - PBS only. 85802-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2716. 85802-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD74 antibody (85802-2-RR, Clone: 250109B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02137-1

  Cytometric bead array standard curve of MP02137-1, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-1-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.

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Informations sur le produit

85802-2-PBS cible CD74 dans les applications de IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD74 Protéine recombinante Eg2716
• Nom complet: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Masse moléculaire calculée: 32 kDa
• Numéro d’acquisition GenBank: NM_001042605.1
• Symbole du gène: Cd74
• Identification du gène (NCBI): 16149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).

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