Anticorps Monoclonal anti-Ch-TOG

Ch-TOG Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H5G1

N° de cat : 67631-1-PBS

Synonymes

CKAP5, 1H5G1, ch TOG, Colonic and hepatic tumor overexpressed gene protein, Cytoskeleton-associated protein 5

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Galerie de données de validation

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  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50224-1

  Cytometric bead array standard curve of MP50224-1, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-2-PBS. Detection antibody: 67631-1-PBS. Standard:Ag28770. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50224-2

  Cytometric bead array standard curve of MP50224-2, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-1-PBS. Detection antibody: 67631-3-PBS. Standard:Ag28770. Range: 6.25-100 ng/mL.

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Informations sur le produit

67631-1-PBS cible Ch-TOG dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ch-TOG Protéine recombinante Ag28770
• Nom complet: cytoskeleton associated protein 5
• Poids moléculaire observé: 220-240 kDa
• Numéro d’acquisition GenBank: BC120869
• Symbole du gène: Ch-TOG
• Identification du gène (NCBI): 9793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

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