Anticorps Monoclonal anti-Chromogranin A

Chromogranin A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A7D11

N° de cat : 60135-2-PBS

Synonymes

CGA, CHGA, 2A7D11, AL-11, Chromogranin-A

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Galerie de données de validation

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  WB analysis of rat adrenal gland using 60135-2-Ig (same clone as 60135-2-PBS)

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (Chromogranin A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 60135-2-Ig (same clone as 60135-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (Chromogranin A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Neuro-2a cells using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50241-2

  Cytometric bead array standard curve of MP50241-2, CHGA/Chromogranin A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60135-2-PBS. Detection antibody: 60135-3-PBS. Standard:Ag19780. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50241-3

  Cytometric bead array standard curve of MP50241-3, CHGA/Chromogranin A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68851-1-PBS. Detection antibody: 60135-2-PBS. Standard:Ag19780. Range: 1.563-100 ng/mL.

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Informations sur le produit

60135-2-PBS cible Chromogranin A dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Chromogranin A Protéine recombinante Ag0807
• Nom complet: chromogranin A (parathyroid secretory protein 1)
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC006459
• Symbole du gène: Chromogranin A
• Identification du gène (NCBI): 1113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

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