Anticorps Recombinant de lapin anti-Citrate synthase

Citrate synthase Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251433D2

N° de cat : 86549-1-RR

Synonymes

CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1

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Galerie de données de validation

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  WB analysis using 86549-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 86549-1-RR

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 86549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86549-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86549-1-RR against Human Citrate synthase were performed. The affinity constant is 0.995 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HepG2, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86549-1-RR cible Citrate synthase dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Citrate synthase Protéine recombinante Ag9117
• Nom complet: citrate synthase
• Masse moléculaire calculée: 466 aa, 52 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010106
• Symbole du gène: Citrate Synthase
• Identification du gène (NCBI): 1431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038).

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