Anticorps Polyclonal de lapin anti-Claudin 1

• Phare
• Validé par KD/KO

Claudin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(162)

N° de cat : 28674-1-AP

Synonymes

Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC

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Galerie de données de validation

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  WB analysis of A431 using 28674-1-AP

  WB result of Claudin 1 antibody (28674-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 1 transfected A431 cells.

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HaCaT using 28674-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HUVEC using 28674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cutané de souris, tissu de cancer de la peau humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HaCaT, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 117 publications below
• IHC: See 33 publications below
• IF: See 40 publications below

Informations sur le produit

28674-1-AP cible Claudin 1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 1 Protéine recombinante Ag29269
• Nom complet: claudin 1
• Masse moléculaire calculée: 211 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC012471
• Symbole du gène: Claudin 1
• Identification du gène (NCBI): 9076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

Publications

Species	Application	Title
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	WB	J Nanobiotechnology; Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis; Authors - Zhiping Wei
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
human	WB	Int J Biol Macromol; Anionic polysaccharides benefit the bioavailability of pork myofibrillar protein gels: Evidence from a perspective of protein absorption and metabolism; Authors - Xia Yu
rat	WB,IF	Phytomedicine; Licorice-regulated gut-joint axis for alleviating collagen-induced rheumatoid arthritis; Authors - Di Yang
mouse,human	WB	Biomed Pharmacother; Bacterial butyrate mediates the anti-atherosclerotic effect of silybin; Authors - Hao-Ran Shen

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