Anticorps Polyclonal de lapin anti-Claudin 5

Claudin 5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29767-1-PBS

Synonymes

CLDN5, AWAL, BEC1, Claudin5, Claudin-5

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Galerie de données de validation

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  WB analysis using 29767-1-AP (same clone as 29767-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 29767-1-AP (same clone as 29767-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Claudin 5 antibody (29767-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29767-1-PBS cible Claudin 5 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 5 Protéine recombinante Ag29044
• Nom complet: claudin 5
• Masse moléculaire calculée: 218 aa, 23 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC032363
• Symbole du gène: CLDN5
• Identification du gène (NCBI): 7122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

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