Anticorps Polyclonal de lapin anti-Claudin 9

Claudin 9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30520-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30520-1-AP (same clone as 30520-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IF Staining of T-47D using 30520-1-AP (same clone as 30520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30520-1-PBS cible Claudin 9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 9 Protéine recombinante Ag29607
• Nom complet: claudin 9
• Masse moléculaire calculée: 217 aa, 23 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC051870
• Symbole du gène: Claudin 9
• Identification du gène (NCBI): 9080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

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