Anticorps Recombinant de lapin anti-Cleaved Caspase 3/P17/P19

Cleaved Caspase 3/P17/P19 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

230527B2

N° de cat : 82707-13-RR

Synonymes

CASP3, Caspase3, 230527B2, CASP 3, CASP-3

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Galerie de données de validation

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  WB analysis using 82707-13-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82707-13-RR (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la staurosporine,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

82707-13-RR cible Cleaved Caspase 3/P17/P19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 17 kDa, 19 kDa
• Numéro d’acquisition GenBank: NM_032991
• Symbole du gène: Caspase 3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publications

Species	Application	Title
mouse	WB,IF	Arch Biochem Biophys; DANCR Knockdown Alleviates Neuroinflammation and Functional Recovery after Spinal Cord Injury via Regulating the ACTN4 / STAT3 Axis; Authors - Bin Xia
mouse	WB	CNS Neurosci Ther; CHOP-Mediated Disruption of Hippocampal Synaptic Plasticity and Neuronal Activity Contributes to Chronic Pain-Related Cognitive Deficits; Authors - Qingsheng Meng
mouse	WB	J Membr Biol; Schisandrin B Improves Mitochondrial Function and Inhibits HT22 Cell Apoptosis by Regulating Sirt3 Protein; Authors - Fei Hu
human	WB	Toxicol Appl Pharmacol; Exploring the mechanism of baicalein on breast cancer based on network pharmacology, molecular docking and in vivo experiments; Authors - Gaotao Zhang
human	WB	Bioorg Chem; Design, synthesis and antitumor activity evaluation of novel modified 18β-glycyrrhetinate derivatives as PPARγ agonists; Authors - Hongyan Lin
human	WB	J Med Chem; Discovery of Novel Oxazolo[4,3-f]purine Derivatives as Antitumor Agents through PPIA Interaction; Authors - Xian-Jia Li

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