Anticorps Monoclonal anti-Cleaved Caspase 3/P17/P19

Cleaved Caspase 3/P17/P19 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(35)

CloneNo.

2F7B8

Avis (1)

N° de cat : 68773-1-Ig

Synonymes

CASP3, Caspase3, 2F7B8, CASP 3, CASP-3

---

Galerie de données de validation

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68773-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 68773-1-Ig

  IP result of anti-Cleaved Caspase 3/P17/P19 (IP:68773-1-Ig, 4ug; Detection:68773-1-Ig 1:5000) with Staurosporine treated Jurkat cells lysate 720 ug.

• 

  IHC staining of human ovary cancer using 68773-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 68773-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 68773-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Jurkat using 68773-1-Ig

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 68773-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BV-2 using 68773-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of Jurkat using 68773-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ).

• 

  IF Staining of HSC-T6 using 68773-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

• 

  FC experiment of Jurkat using 68773-1-Ig

  1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

• 

  FC experiment of Jurkat using 68773-1-Ig

  1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

• 

  FC experiment of BV-2 using 68773-1-Ig

  1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A2780, cellules HepG2, cellules HSC-T6, cellules Ramos
• Résultats positifs en IP: cellules Jurkat traitées à la staurosporine,
• Résultats positifs en IHC: tissu de lymphome humain, cellules Jurkat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en IF/ICC: 1 μM Staurosporine (1/3/6 hour) treated Jurkat cells, 50 μM Cisplatin (18 hours) treated BV-2 cells, 1 μM Staurosporine (1/3/6 hour) treated HSC-T6 cells
• Résultats positifs en FC (Intra): 1 μM Staurosporine (3 hours) treated Jurkat cells, 50 μM Cisplatin（18h）treated BV-2 cells

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Informations sur le produit

68773-1-Ig cible Cleaved Caspase 3/P17/P19 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 17 kDa, 19 kDa
• Numéro d’acquisition GenBank: NM_032991
• Symbole du gène: Caspase 3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publications

Species	Application	Title
mouse	WB	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
mouse	WB	Clin Transl Med; PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction; Authors - Hao Zhang
human	WB	Int J Biol Macromol; A functionalized lignin dual network nanoplatform for enhanced cancer therapy by triggering reactive oxygen species self-circulation; Authors - Wenda Wang
human	WB	Int J Biol Macromol; Schizophyllum commune fruiting body polysaccharides inhibit glioma by mediating ARHI regulation of PI3K/AKT signalling pathway; Authors - Shi-Xing Zheng
	IF	Mol Neurobiol; FGF21 Alleviates Hypoxic-Ischemic White Matter Injury in Neonatal Mice by Mediating Inflammation and Oxidative Stress Through PPAR-γ Signaling Pathway; Authors - Mingchu Fang
mouse	WB	J Asian Nat Prod Res; Taxifolin ameliorates the D-galactose-induced aging of mouse hippocampal neurons HT-22 cells through modulating SIRT1/p53 and PI3K/AKT signaling pathways; Authors - Xing-Long Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Molly (Verified Customer) (10-03-2024)

Worked well in IF on primary rat embryonic neuron/astrocyte co-culture at a range of dilutions.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Rat primary neuron/astrocyte culture

{{ptg:RelatedPrimaryAntibodies}}