Anticorps Recombinant de lapin anti-Cleaved PARP1 (Asp214)

Cleaved PARP1 (Asp214) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243142B10

N° de cat : 85175-5-RR

Synonymes

PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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Galerie de données de validation

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  WB analysis of HeLa using 85175-5-RR

  Staurosporine treated HeLa cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85175-5-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Staurosporine treated HeLa cells, cellules Jurkat traitées à la staurosporine

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85175-5-RR cible Cleaved PARP1 (Asp214) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: poly (ADP-ribose) polymerase 1
• Masse moléculaire calculée: 1014 aa, 113 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC037545
• Symbole du gène: PARP1
• Identification du gène (NCBI): 142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.

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