Anticorps Recombinant de lapin anti-Clusterin

Clusterin Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241277D4

N° de cat : 84067-4-PBS

Synonymes

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

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Galerie de données de validation

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  WB analysis of human plasma using 84067-4-RR (same clone as 84067-4-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84067-4-RR (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84067-4-RR (same clone as 84067-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (84067-4-RR, Clone: 241277D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84067-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human Clusterin were performed. The affinity constant is 6.91 nM.

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Informations sur le produit

84067-4-PBS cible Clusterin dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: clusterin
• Masse moléculaire calculée: 449 aa, 53 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC010514
• Symbole du gène: CLU
• Identification du gène (NCBI): 1191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

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