Anticorps Recombinant de lapin anti-Tissue Factor/CD142

Tissue Factor/CD142 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240784H6

N° de cat : 83776-4-PBS

Synonymes

CD142, Coagulation factor III, F3, Tissue factor, 240784H6

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Galerie de données de validation

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  WB analysis of HepG2 using 83776-4-RR (same clone as 83776-4-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83776-4-RR (same clone as 83776-4-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83776-4-RR (same clone as 83776-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83776-4-RR (Tissue Factor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83776-4-RR (same clone as 83776-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Tissue Factor antibody (83776-4-RR, Clone: 240784H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83776-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 83776-4-PBS against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

• 

  Affinity and Kinetic Characterization of 83776-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83776-4-RR against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

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Informations sur le produit

83776-4-PBS cible Tissue Factor/CD142 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Tissue Factor/CD142 Protéine recombinante Eg1052
• Nom complet: coagulation factor III (thromboplastin, tissue factor)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_001993.5
• Symbole du gène: F3
• Identification du gène (NCBI): 2152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

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