Anticorps Monoclonal anti-Cofilin

• Phare
• Validé par KD/KO

Cofilin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6A2

N° de cat : 66057-1-PBS

Synonymes

CFL1, Cofilin1, Cofilin, non-muscle isoform, Cofilin 1, 1G6A2

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Galerie de données de validation

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  WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cofilin antibody (66057-1-Ig, Clone: 1G6A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66057-1-PBS cible Cofilin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cofilin Protéine recombinante Ag18044
• Nom complet: cofilin 1 (non-muscle)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC012318
• Symbole du gène: Cofilin
• Identification du gène (NCBI): 1072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

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