Anticorps Polyclonal de lapin anti-Coilin

Coilin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10967-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10967-1-AP (same clone as 10967-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10967-1-AP (same clone as 10967-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10967-1-AP (same clone as 10967-1-PBS)

  IP result of anti-Coilin (IP:10967-1-AP, 4ug; Detection:10967-1-AP 1:10000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10967-1-AP (same clone as 10967-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10967-1-AP (same clone as 10967-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10967-1-AP (same clone as 10967-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10967-1-AP (same clone as 10967-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Coilin antibody (10967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 10967-1-AP (same clone as 10967-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Coilin (10967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10967-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10967-1-PBS cible Coilin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Coilin Protéine recombinante Ag1416
• Nom complet: coilin
• Masse moléculaire calculée: 576 aa, 63 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC010385
• Symbole du gène: COIL
• Identification du gène (NCBI): 8161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

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