Anticorps Monoclonal anti-Collagen Type I

Collagen Type I Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D5E8

N° de cat : 66761-1-PBS

Synonymes

COL1, COL1A2, 3D5E8, Alpha-2 type I collagen, col I

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Galerie de données de validation

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  WB analysis of rat skin using 66761-1-Ig (same clone as 66761-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66761-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66761-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Mouse tail using 66761-1-Ig (same clone as 66761-1-PBS)

  Mouse tail tissue were subjected to SDS PAGE followed by western blot with 66761-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66761-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig skin using 66761-1-Ig (same clone as 66761-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66761-1-Ig (Collagen Type I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66761-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66761-1-PBS cible Collagen Type I dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Collagen Type I Protéine recombinante Ag6281
• Nom complet: collagen, type I, alpha 2
• Masse moléculaire calculée: 1366 aa, 130 kDa
• Poids moléculaire observé: 140 kDa, 70-80 kDa
• Numéro d’acquisition GenBank: BC054498
• Symbole du gène: COL1A2
• Identification du gène (NCBI): 1278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. When MMPs degrade type I collagen, the major degradation products are the typical ¾ and ¼ collagen fragments with molecular weights of ~ 50-70 kDa, and ~ 25 (PMID: 8910479).

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