Anticorps Recombinant de lapin anti-Collagen Type I

Collagen Type I Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

250534B1

N° de cat : 86093-1-RR

Synonymes

COL1A1, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I, col1

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Galerie de données de validation

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  WB analysis of SK-N-SH using 86093-1-RR

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 86093-1-RR (COL1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86093-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86093-1-RR against Human Collagen Type I were performed. The affinity constant is 4.37 nM.

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Applications testées

• Résultats positifs en WB: SK-N-SH cells,
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86093-1-RR cible Collagen Type I dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Collagen Type I Protéine recombinante Ag27389
• Nom complet: collagen, type I, alpha 1
• Masse moléculaire calculée: 1464 aa, 139 kDa
• Poids moléculaire observé: 140 kDa, 200kDa
• Numéro d’acquisition GenBank: BC036531
• Symbole du gène: COL1A1
• Identification du gène (NCBI): 1277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).

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