Anticorps Monoclonal anti-COL5A1

COL5A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C2C2

N° de cat : 67604-1-PBS

Synonymes

2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

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Galerie de données de validation

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  WB analysis of HT-1080 using 67604-1-Ig (same clone as 67604-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 67604-1-Ig (same clone as 67604-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67604-1-Ig (same clone as 67604-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67604-1-PBS cible COL5A1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: native protein
• Nom complet: collagen, type V, alpha 1
• Masse moléculaire calculée: 184 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: NM_000093
• Symbole du gène: COL5A1
• Identification du gène (NCBI): 1289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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