Anticorps Monoclonal anti-COL6A1

COL6A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3H10B1

N° de cat : 66824-1-PBS

Synonymes

COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 66824-1-Ig (same clone as 66824-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66824-1-Ig (same clone as 66824-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66824-1-Ig (same clone as 66824-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66824-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66824-1-PBS cible COL6A1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COL6A1 Protéine recombinante Ag11809
• Nom complet: collagen, type VI, alpha 1
• Masse moléculaire calculée: 1028 aa, 109 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC052575
• Symbole du gène: COL6A1
• Identification du gène (NCBI): 1291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

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